Composite

Part:BBa_K4614108:Design

Designed by: Qianhui Sun   Group: iGEM23_CAU-China   (2023-10-06)


T7 Promoter-RBS-LuxI-B0015 Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This pathway is used for expression on low-copy-number plasmids. The strong 17 promoter could lead to issues such as inclusion body formation.



Source

The T7, B0015 and the RBS sequences can all be found in the part library. The luxI gene can be obtained by designing primers and conducting PCR from Vibrio fischeri. Our team's luxI gene was generously provided as plasmids from ShanghaiTech_China 2023.

References